Chemical reagents, kits, and other products used for DNA sequencing; includes sequencing and pre-sequencing kits, purification kits, buffers, standards, etc.
The KAPA EvoPrep Kit is a high-performance solution for Next-Generation Sequencing (NGS) DNA library preparation, designed to simplify workflows and reduce errors. The kit delivers excellent sequencing performance across various research applications, including whole-genome and targeted sequencing, with enhanced library conversion rates.
High-performance DNA library preparation kit
Streamlined, automation-friendly workflow
Compatible with all Illumina sequencing instruments
Validated for a variety of challenging sample inputs
High conversion rates up to 100%
Flexible input amount ranging from 0.1 ng to 500 ng
Storage temperature between -15 °C and -20 °C
Includes additional components available separately
For research use only, not for diagnostic procedures
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CleanDTR is an efficient, paramagnetic bead-based system, designed to remove unincorporated dye terminators from Sanger sequencing reactions prior to capillary electrophoresis. The process involves just three simple steps: bind, wash, and elute.
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The novel NEBNext Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext Small RNA Library Prep Set for Illumina (Set 1) includes adaptors and multiplex primers with 12 indices, to enable multiplexing.
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Indexes come as pre-mixes, and the forward and reverse primers are provided at 5 M total concentration (2.5 M each). These indexes can be used to barcode any library containing TruSeq adapters and are compatible with the Zymo-Seq RiboFree™ Total RNA Library Kit (R3000 & R3003). Zymo-Seq™ index primers generate sequencing libraries that are compatible with all Illumina platforms.
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This module is part of the Ultra II workflow, and is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module, for Illumina-compatible library construction. The NEBNext Ultra II Ligation Module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module or the NEBNext Ultra II FS DNA Module. This module is also compatible with some Oxford Nanopore Technologies workflows.
Optimized to ligate DNA adaptors for Illumina sequencing to end-repaired, dA-tailed DNA fragments
Compatible with some Oxford Nanopore Technologies workflows
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The only difference is that each contains either 100% unmodified cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines. Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine modifications.
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The Human HCT116 DKO Non-methylated DNA is purified from cells that contain genetic knockouts of both DNA methyltransferasesDNMT1 (-/-) and DNMT3b (-/-). The DNA derived from HCT116 DKO cells has a low level of DNA methylation and can be used as a control for DNA methylation analysis. The Human HCT116 DKO Methylated DNA is purified HCT116 DKO DNA that has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by M.SssI methyltransferase2 (EC 2.1.1.37) and can be used as a positive control for DNA methylation analysis.
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The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process - simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 l. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
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